Table 1 Gene and primer information used in this study.
From: Curcumin and resveratrol inhibit nuclear factor-kappaB-mediated cytokine expression in adipocytes
Gene | GenBank no. | 5' primer – top sequence 3' primer – bottom sequence | Intron flanked | Base pairs amplified |
|---|---|---|---|---|
COX-2 | NM_011198 | TGGGGTGATGAGCAACTATT AAGGAGCTCTGGGTCAAACT | 7 | 132 |
IL-1β | NM_008361 | GACCTTCCAGGATGAGGACA AGCTCATATGGGTCCGACAG | 3 | 183 |
IL-6 | NM_031168 | AGTTGCCTTCTTGGGACTGA CAGAATTGCCATTGCACAAC | 2 | 191 |
TNFα | NM_013693 | ACGGCATGGATCTCAAAGAC GTGGGTGAGGAGCACGTAGT | none | 116 |
β-actin | NM_007393 | CCTGAACCCTAAGGCCAACC CAGCTGTGGTGGTGAAGCTG | 3 | 287 |
leptin | NM_008493 | TGACACCAAAACCCTCATCA TCATTGGCTATCTGCAGCAC | 2 | 213 |
F4/80 | NM_010130 | GCTGTGAGATTGTGGAAGCA CTGTACCCACATGGCTGATG | 15 | 135 |
Mac-1 | NM_008401 | AAGGATTCAGCAAGCCAGAA TAGCAGGAAAGATGGGATGG | 20 | 136 |