The effect of leucine on oxygen consumption in (A) C2C12 cells and (B) 3T3-L1 adipocytes. Cells were pre-treated with or without 0.5 mM leucine and data were presented as the dissolved oxygen concentration in perfusion fluid RPMI 1640. The "blank" depicts O2 in the absence of cells on the coverslip. The figures depict polynomial trendlines superimposed upon the data points. Initial rates were compared using data from the first 3 mins data of oxygen consumption in both cells types.