Figure 9
TLC-fingerprinting of the various fractions of the butanolic extract of hibiscus sabdariffa. Eluent: mixture of ethyl acetate/icy acetic acid/formic acid/water (100:11:11:26). Detection: in natural light without revealing; Spots: 1 μg/μl of butanolic extract followed by various fractions of elution at the same concentration: F1-4, F6-8, F9-15, F16-18, F19-23, F24-32, F33-42 and F43-67. Support: Silicagel 60 F254 Merck;