Figure 3

Effects of EPA and COX-2 inhibition on secreted PGE ₂ levels from 3T3-L1 adipocytes. 3T3 L-1 adipocytes were grown 6–7 days post-confluence, and starved for 24 h in serum-free media containing FA-free BSA. Fatty acids were incubated in media containing FA-free BSA for two hours at 37°C in a shaking water bath prior to treatment. Treatment consisted of EPA (50 and 150 μM), EPA (150 μM) + CI (5 μM), CI (5 μM) and EPA (150 μM) in media without cells. After 48 hours, 2 mL of media was removed and stored at -80°C. Media PGE₂ levels were analyzed using EIA as described in the Materials and Methods. Results represent the mean ± SEM with a number of treatments n = 3–4. Values labeled with different letters are significantly different (p < 0.05). Values with the same letters do not differ significantly. * Denotes culture media exposed to cell culture conditions without cells.