Figure 6

Immunoblot analysis of tight junction (TJ) proteins in the whole cell extracts and detergent-insoluble fractions of cecal and colonic mucosa of the OLETF and LETO rats fed the standard and high fat diets. The mucosa was collected at the end of the experiment (at 16 weeks) and immunoblotted for occludin, claudin-1, claudin-3, junctional adhesion molecules-1 (JAM-1), and β-actin. The immunoblot was representative of 8 rats.