Table 4 Effect of dietary milk phospholipids in the form of commercially-prepared PC-700 on liver and fecal lipid parameters in mice given an intragastric gavage of radioactively-labelled cholesterol and sitostanol after 3 or 5 weeks on high-fat diet.
| Â | 3 weeks | 5 weeks | Combined | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Â | HF | HFPC-700 | difference | HF | HFPC-700 | difference | HF | HFPC-700 | difference |
| Â | (n = 10) | (n = 9) | Â | (n = 9) | (n = 10) | Â | (n = 19) | (n = 19) | Â |
Liver | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Total lipid (mg/organ) | 103 ± 10 | 80 ± 5 | -22% | 148 ± 18 | 82 ± 7 ** | -45% | 124 ± 11 | 81 ± 4 ** | -35% |
Cholesterol (μmol/organ) | 30 ± 3 | 17 ± 1 ** | -45% | 37 ± 4 | 16 ± 2 *** | -57% | 34 ± 3 | 16 ± 1 *** | -51% |
Triglyceride (μmol/organ) | 80 ± 13 | 52 ± 8 | -35% | 110 ± 14 | 41 ± 8 *** | -63% | 94 ± 10 | 46 ± 6 *** | -51% |
[14C]cholesterol (dpm/organ) | 5377 ± 902 | 2150 ± 248 ** | -60% | 6390 ± 1006 | 2268 ± 305 *** | -65% | 5857 ± 664 | 2212 ± 194 *** | -62% |
[3H]sitostanol (dpm/organ) | 403 ± 76 | 264 ± 31 | -34% | 310 ± 49 | 206 ± 28 | -33% | 359 ± 46 | 234 ± 21 * | -35% |
Feces | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Total lipid (mg/mouse/day) | 15.5 ± 1.4 | 22.3 ± 1.7 ** | +44% | 12.3 ± 1.1 | 18.8 ± 2.0 * | +53% | 14.0 ± 1.0 | 20.4 ± 1.3 *** | +46% |
Cholesterol (μmol/mouse/day) | 6.8 ± 0.7 | 7.9 ± 0.6 | +17% | 6.8 ± 0.5 | 8.4 ± 0.5 * | +24% | 6.8 ± 0.4 | 8.2 ± 0.4 * | +21% |
Triglyceride (μmol/mouse/day) | 4.6 ± 1.1 | 6.1 ± 1.5 | +33% | 2.3 ± 0.6 | 3.7 ± 1.0 | +62% | 3.5 ± 0.7 | 4.9 ± 0.9 | +38% |
[14C]cholesterol (dpm/mouse/day) | 10289 ± 675 | 12163 ± 437 * | +18% | 9075 ± 770 | 11208 ± 550 * | +24% | 9714 ± 515 | 11660 ± 364 ** | +20% |
[3H]sitostanol (dpm/mouse/day) | 17778 ± 962 | 19490 ± 981 | +10% | 18173 ± 907 | 17552 ± 618 | -3% | 17965 ± 647 | 18470 ± 596 | +3% |