Figure 1

Bilberry extracts inhibited 3T3-L1 adipocyte differentiation. (A) 3T3-L1 preadipocytes were induced to differentiate in the presence of DMSO or 10, 50 or 100 μ g/mL of bilberry extracts for six days. On day 7, the cells were stained with Oil Red O and photographed. The lipid content in these cells was quantitatively determined as described in the Methods and Procedures. Data are expressed as the ratio of samples/control (DMSO treated cells) levels (n = 3). The results are shown as the mean ± S.D. (* p < 0.05, ** p < 0.001, Welch's t-test). (B) 3T3-L1 preadipocytes were induced to differentiate in the presence of DMSO or 100 μ g/mL of bilberry extracts. The Ppar γ and adiponectin mRNA levels on day 7 of differentiation were determined by the real-time RT-PCR (n = 3). β -actin was used as the reference gene (** p < 0.001, Welch's t-test).