From: Changes in white adipose tissue metabolism induced by resveratrol in rats
SYBR® Green RT-PCR: | |||
---|---|---|---|
Primers | Sense primer | Antisense primer | Annealing Temperature |
ACC | 5'-GGACCACTGCATGGAATGTTAA-3' | 5'-TGAGTGACTGCCGAAACATCTC-3' | 60.6°C |
FAS | 5'-AGC CCC TCA AGT GCA CAG TG-3' | 5'-TGCCAATGTGTTTTCCCTGA-3' | 60.0°C |
SREBP-1c | 5'-GCGGACGCAGTCTGGG-3' | 5'-ATGAGCTGGAGCATGTCTTCAAA-3' | 67.3°C |
ATGL | 5'-CACTTTAGCTCCAAGGATGA-3' | 5'-TGGTTCAGTAGGCCATTCCT-3' | 62.0°C |
Perilipin | 5'-AGAGGAGACAGATGAGGAGGAAG -3' | 5'-AGATGGTGTTCTGCAGAGTCTTC -3' | 63.9°C |
LPL | 5'- CAGCTGGGCCTAACTTTGAG -3' | 5'- CCTCTCTGCAATCACACGAA -3' | 61.5°C |
PPARγ | 5'-ATTCTGGCCCACCAACTTCGG -3' | 5'-TGGAAGCCTGATGCTTTATCCCCA -3' | 64.5°C |
β-Actin | 5'-ACGAGGCCCAGAGCAAGAG-3' | 5'-GGTGTGGTGCCAGATCTTCTC-3' | 60.0°C |