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Table 1 Primers and annealing temperatures for PCR amplification of each gene studied.

From: Changes in white adipose tissue metabolism induced by resveratrol in rats

SYBR® Green RT-PCR:

Primers

Sense primer

Antisense primer

Annealing Temperature

ACC

5'-GGACCACTGCATGGAATGTTAA-3'

5'-TGAGTGACTGCCGAAACATCTC-3'

60.6°C

FAS

5'-AGC CCC TCA AGT GCA CAG TG-3'

5'-TGCCAATGTGTTTTCCCTGA-3'

60.0°C

SREBP-1c

5'-GCGGACGCAGTCTGGG-3'

5'-ATGAGCTGGAGCATGTCTTCAAA-3'

67.3°C

ATGL

5'-CACTTTAGCTCCAAGGATGA-3'

5'-TGGTTCAGTAGGCCATTCCT-3'

62.0°C

Perilipin

5'-AGAGGAGACAGATGAGGAGGAAG -3'

5'-AGATGGTGTTCTGCAGAGTCTTC -3'

63.9°C

LPL

5'- CAGCTGGGCCTAACTTTGAG -3'

5'- CCTCTCTGCAATCACACGAA -3'

61.5°C

PPARγ

5'-ATTCTGGCCCACCAACTTCGG -3'

5'-TGGAAGCCTGATGCTTTATCCCCA -3'

64.5°C

β-Actin

5'-ACGAGGCCCAGAGCAAGAG-3'

5'-GGTGTGGTGCCAGATCTTCTC-3'

60.0°C

  1. ACC, acetyl-CoA carboxylase; FAS, fatty acid synthase; SREBP-1c, sterol-regulatory element binding protein-1c; ATGL, adipose triglyceride lipase; LPL, lipoprotein lipase; PPAR, peroxisome proliferator-activated receptor.