Determination of intracellular fat content in adipocytes differentiated for 7 days with varying concentrations of rosiglitazone. (A) Oil Red O quantification according to the Materials and Methods section. (B) Fat droplet number (Spot Count/Object) and droplet intensity (Spot Avg Intensity), using the Cellomics HCS Reader. Neutral lipids were stained with BODIPY® 493/503. Results are expressed as percentage of values obtained with 100 μM rosiglitazone (mean ± SEM, n = 8).