Figure 3

Renal expression of IκBα, TNF-α, IL-1β, p-NF-κB and NF-κB. (A) Representative Western blots showing specific bands for IκBα, TNF-α, IL-1β and β-actin as an internal control. Equal amounts of protein sample obtained from whole kidney homogenates were applied in each lane. These bands are representative of five separate experiments. (B-D) Graphical representation of data from Western blots analyses. The mean density values of IκBα, TNF-α and IL-1β are expressed as ratios relative to that of β-actin. Values are means ± SEM. **p < 0.05 vs C; ^##p < 0.05 vs D for IκBα, **p < 0.01 vs C; ^##p < 0.01 vs D for TNF-α and IL-1β. (E) Representative Western blots showing specific bands for phosphorylated NF-κB, NF-κB as an internal control and lamin A for equal loading of nuclear sample. (F) Densitometric data of protein analysis. The mean density values of p-NF-κB are expressed as ratios relative to that of NF-κB. C, age-matched normal rats; D, diabetic-treated rats administered with vehicle; Cur, diabetic rats treated with curcumin 100 mg/kg/day. ** p < 0.01 vs. C; ^## p < 0.05 vs. D based on one way ANOVA followed by Tukey's test.