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Table 1 Mean values of fatty acid composition (%), lipid peroxidation parameters and antiradical properties of high-fat diet (fresh soybean oil, FSO; oxidized soybean oil, OSO and margarine, MG)

From: The intake of high fat diet with different trans fatty acid levels differentially induces oxidative stress and non alcoholic fatty liver disease (NAFLD) in rats

 

Supplemented high-dietary fat

 

FSO

OSO

MG

Fatty acids (%)

   

8:0

nd

nd

0.26 ± 0.00##

10:0

nd

nd

0.26 ± 0.00##

12:0

nd

nd

3.16 ± 0.01##

14:0

0.08 ± 0.002

0.08 ± 0.01

1.84 ± 0.03##

14:1

0.02 ± 0.00

0.02 ± 0.009

0.04 ± 0.00#

16:0

10.96 ± 0.06

12.08 ± 0.01**

30.33 ± 0.04##

trans- 16:1 n-7

0.02 ± 0.00

0.024 ± 0.006

0.03 ± 0.00 #

cis- 16:1 n-7

0.09 ± 0.00

0.11 ± 0.00**

0.13 ± 0.00#

17:0

0.29 ± 0.02

0.28 ± 0.01

0.20 ± 0.01##

17:1

0.08 ± 0.02

0.08 ± 0.00

0.05 ± 0.01##

18:0

4.82 ± 0.04

3.93 ± 0.01**

4.8 ± 0.01##

trans- 18:1 n-9

nd

0.117 ± 0.01**

1.78 ± 0.13 ##

trans- 18:1 n-7

nd

nd

nd

cis- 18:1 n-9

21.96 ± 0.2

25.22 ± 0.02**

30.1 ± 0.13##

cis- 18:1 n-7

1.29 ± 0.05

1.71 ± 0.01**

0.73 ± 0.00##

18:2 n-6 (t9. t12)

0.07 ± 0.00

0.138 ± 0.01**

0.096 ± 0.001 #

18:2 n-6 (t9. c12)

nd

0.054 ± 0.003**

0.052 ± 0.004

18:2 n-6 (c9. t12)

0.09 ± 0.01

0.288 ± 0.1**

0.2 ± 0.01 ##

18:2 n-6 (c9. c12)

50.75 ± 0.04

48.12 ± 0.01**

21.73 ± 0.3##

cis- 18:3 n-6

0.19 ± 0.00

0.40 ± 0.05**

0.14 ± 0.00##

trans-18:3 n-3

0.02 ± 0.00

0.366 ± 0.01**

0.016 ± 0.011 ##

cis- 18:3 n-3

7.65 ± 0.1

4.76 ± 0.02**

2.55 ± 0.02##

18:2 (c9. t11)

0.024 ± 0.001

0.099 ± 0.003**

0.068 ± 0.00 ##

18:2 (t10. c12)

0.013 ± 0.001

0.056 ± 0.004**

0.042 ± 0.00 #

20:0

0.43 ± 0.01

0.43 ± 0.002

0.35 ± 0.00##

trans- 20:1 n-9

0.026 ± 0.00

0.199 ± 0.006**

0.06 ± 0.00 ##

20:1 n-9

0.24 ± 0.01

0.2 ± 0.080*

0.18 ± 0.02#

20:2 n-9

0.08 ± 0.003

0.09 ± 0.002

0.02 ± 0.00##

20:3 n-6

0.03 ± 0.00

0.04 ± 0.02**

0.02 ± 0.00

20:4 n-6

0.03 ± 0.00

0.03 ± 0.00

0.01 ± 0.00#

22:0

0.06 ± 0.00

0.067 ± 0.00*

0.03 ± 0.00##

ΣSFA

16.22 ± 0.13

16.9 ± 0.03**

41.42 ± 0.1 ##

Σcis MUFA

23.6 ± 0.3

27.47 ± 0.1**

31.25 ± 0.13 ##

Σ cis PUFA

59.36 ± 0.12

54.21 ± 0.1**

24.81 ± 0.03 ##

Total TFAs

0.226 < 1

1 < 1.23 < 2

2.4 > 2

Lipid peroxidation

   

Oxidative stability index (h)

3.74 ± 0.01

0.67 ± 0.04**

4.27 ± 0.63##

Peroxide value (meq O2/kg)

2.66 ± 0.00

6 ± 0.00**

17.33 ± 0.94##

p-anisidine value

2.13 ± 0.7

7.5 ± 2.2**

2.61 ± .024##

k232 (conjugated dienes)

2.77 ± 0.10

4.26 ± 0.04**

3.9 ± 0.07

k270

1.11 ± 0.05

4.01 ± 0.05**

2.54 ± 0.15##

Antiradical ability: DPPH (%)

93.12 ± 0.06

50.16 ± 2.88**

77.91 ± 0.5##

  1. nd: not detected.
  2. Values are given as mean ± SD (n = 3). FSO: Fresh soybean oil, OSO: oxidized soybean oil,
  3. MG: margarine.
  4. *p < .05, OSO vs. FSO; **p < .01 OSO vs. FSO. #p < .05, MG vs. OSO; ##p < .05 MG vs. OSO. Comparison between supplemented-diet compositions was made using unpaired Student t test.