Table 1 Analysis of islet and MIN6 cell mRNA expression

Gene name

Control

Rosi

EGCG

Control

EGCG

Palmitate

EGCG+Palmitate

Forward

Reverse

Cpt-1

100 ± 21

36 ± 12*

25 ± 9*

100 ± 10

79 ± 3

1182 ± 90*

615 ± 46#

5'-GATCTACAATTCCCCTCTGC-3'

5'-CCTCTGTGGTACACGACAAT-3'

Glut-2

100 ± 10

78 ± 19

70 ± 24

100 ± 2

102 ± 9

33 ± 1*

32 ± 3

5'-TGGCTTTCACTGTCTTCACT-3'

5'-GTGCCATTGACGTCATAGTT-3'

Glucokinase

100 ± 9

123 ± 5

83 ± 10

100 ± 5

88 ± 13

89 ± 5

60 ± 8

5'-GGCACGAAGACATAGACAAG-3'

5'-CACCACATCCATCTCAAAGT-3'

Ddit3

100 ± 19

111 ± 16

61 ± 5*

100 ± 19

74 ± 14

646 ± 25*

485 ± 73#

5'-TCTGTCTCTCCGGAAGTGTA-3'

5'-CTGGTCTACCCTCAGTCCTC-3'

Ppp1r15a

100 ± 7

98 ± 4

71 ± 5*

100 ± 6

91 ± 7

1004 ± 57*

628 ± 50#

5'-TTCTATCTCCTGTCCCCACT-3'

5'-TACCAGAGACAGGGGTAGGT-3'

Cdkn1a

100 ± 23

88 ± 19

43 ± 11*

100 ± 18

150 ± 43

439 ± 51*

579 ± 26

5'-ATTGCTCAGACCTGTGAAGA-3'

5'-AGCAGCAGATCACCAGATTA-3'

Pdx1

100 ± 16

90 ± 8

86 ± 10

-

-

-

-

5'-CCCTGAGCTTCTGAAAACTT-3'

5'-AGCCCAGGTTGTCTAAATTG-3'

Insulin 1

100 ± 15

126 ± 23

58 ± 10*

100 ± 3

103 ± 5

64 ± 4*

51 ± 2#

5'-AGGTAGGCAACCGTGTAAAT-3'

5'-ACCTTCCTCTCAGGAGTCAG-3'

Glucagon

100 ± 11

216 ± 30*

46 ± 7*

-

-

-

-

5'-GATTTTGTGCAGTGGTTGAT-3'

5'-ACTTCTTCTGGGAAGTCTCG-3'

Somatostatin

100 ± 13

116 ± 21

39 ± 4*

-

-

-

-

5'-TCTGGAAGACATTCACATCC-3'

5'-CAATTTCTAATGCAGGGTCA-3'