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Table 1 Analysis of islet and MIN6 cell mRNA expression

From: Diet supplementation with green tea extract epigallocatechin gallate prevents progression to glucose intolerance in db/db mice

  db/db islets MIN6 cells   
Gene name Control Rosi EGCG Control EGCG Palmitate EGCG+Palmitate Forward Reverse
Cpt-1 100 ± 21 36 ± 12* 25 ± 9* 100 ± 10 79 ± 3 1182 ± 90* 615 ± 46# 5'-GATCTACAATTCCCCTCTGC-3' 5'-CCTCTGTGGTACACGACAAT-3'
Glut-2 100 ± 10 78 ± 19 70 ± 24 100 ± 2 102 ± 9 33 ± 1* 32 ± 3 5'-TGGCTTTCACTGTCTTCACT-3' 5'-GTGCCATTGACGTCATAGTT-3'
Glucokinase 100 ± 9 123 ± 5 83 ± 10 100 ± 5 88 ± 13 89 ± 5 60 ± 8 5'-GGCACGAAGACATAGACAAG-3' 5'-CACCACATCCATCTCAAAGT-3'
Ddit3 100 ± 19 111 ± 16 61 ± 5* 100 ± 19 74 ± 14 646 ± 25* 485 ± 73# 5'-TCTGTCTCTCCGGAAGTGTA-3' 5'-CTGGTCTACCCTCAGTCCTC-3'
Ppp1r15a 100 ± 7 98 ± 4 71 ± 5* 100 ± 6 91 ± 7 1004 ± 57* 628 ± 50# 5'-TTCTATCTCCTGTCCCCACT-3' 5'-TACCAGAGACAGGGGTAGGT-3'
Cdkn1a 100 ± 23 88 ± 19 43 ± 11* 100 ± 18 150 ± 43 439 ± 51* 579 ± 26 5'-ATTGCTCAGACCTGTGAAGA-3' 5'-AGCAGCAGATCACCAGATTA-3'
Pdx1 100 ± 16 90 ± 8 86 ± 10 - - - - 5'-CCCTGAGCTTCTGAAAACTT-3' 5'-AGCCCAGGTTGTCTAAATTG-3'
Insulin 1 100 ± 15 126 ± 23 58 ± 10* 100 ± 3 103 ± 5 64 ± 4* 51 ± 2# 5'-AGGTAGGCAACCGTGTAAAT-3' 5'-ACCTTCCTCTCAGGAGTCAG-3'
Glucagon 100 ± 11 216 ± 30* 46 ± 7* - - - - 5'-GATTTTGTGCAGTGGTTGAT-3' 5'-ACTTCTTCTGGGAAGTCTCG-3'
Somatostatin 100 ± 13 116 ± 21 39 ± 4* - - - - 5'-TCTGGAAGACATTCACATCC-3' 5'-CAATTTCTAATGCAGGGTCA-3'
  1. Analysis of mRNA expression in islets of Langerhans isolated from control db/db mice and db/db mice receiving dietary supplementation with 1% (w/w) EGCG or 0.0021% (w/w) rosiglitazone (Rosi) for 10 weeks. The mRNA levels of the indicated genes were normalized to the levels of β-actin mRNA and expressed as mean percent ± SEM of the control group, n = 4. * denotes P < 0.05 for a chance difference vs controls and # denotes P < 0.05 for a chance difference vs palmitate using one-way ANOVA. MIN6 cells were exposed in vitro for 48 hrs to EGCG (20 μM), palmitate (0.5 mM) or a combination thereof
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Nutrition & Metabolism

ISSN: 1743-7075

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