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Table 1 Respiratory parametersa of isolated rat liver mitochondria with pyruvate, D-lactate, or L-lactate or as combination.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb

State 4

State 3

RCR

ADP/O

No substrate

3.24 ± 0.28

3.37 ± 0.34

1.04 ± 0.02

 

Cocktailc

4.11 ± 0.13

30.12 ± 2.02*

7.33 ± 0.46*

2.87 ± 0.09

Pyruvate

5.01 ± 0.56

19.17 ± 2.56*

3.78 ± 0.11*

2.56 ± 0.04

D-lactate

3.82 ± 0.24

14.11 ± 1.13*

3.69 ± 0.11*

2.47 ± 0.11

L-lactate

4.22 ± 0.19

15.02 ± 0.98*

3.56 ± 0.15*

2.30 ± 0.24

Pyruvate + LLA

6.20 ± 0.41

21.40 ± 1.59*

3.45 ± 0.06*

-

Pyruvate + DLA

3.86 ± 0.23

17.58 ± 2.21*

4.56 ± 0.51*

-

LLA + DLA

3.76 ± 0.21

11.78 ± 0.41

3.14 ± 0.07*

-

  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate), DLA or LLA (5 mM + 2.5 mM malate), and LLA+DLA (2.5 mM LLA and 2.5 mM DLA+ 2.5 mM malate).
  3. cCocktail contains 62.5 μM each of malate, glutamate, alpha-ketoglutarate and pyruvate.
  4. State 4: The respiration state without ADP.
  5. State 3: The respiration state with the addition of ADP.
  6. RCR, Respiratory Control Ratio (state 3 rate:state 4 rate).
  7. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  8. *Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test, α < 0.05.