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Table 1 Respiratory parametersa of isolated rat liver mitochondria with pyruvate, D-lactate, or L-lactate or as combination.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb State 4 State 3 RCR ADP/O
No substrate 3.24 ± 0.28 3.37 ± 0.34 1.04 ± 0.02  
Cocktailc 4.11 ± 0.13 30.12 ± 2.02* 7.33 ± 0.46* 2.87 ± 0.09
Pyruvate 5.01 ± 0.56 19.17 ± 2.56* 3.78 ± 0.11* 2.56 ± 0.04
D-lactate 3.82 ± 0.24 14.11 ± 1.13* 3.69 ± 0.11* 2.47 ± 0.11
L-lactate 4.22 ± 0.19 15.02 ± 0.98* 3.56 ± 0.15* 2.30 ± 0.24
Pyruvate + LLA 6.20 ± 0.41 21.40 ± 1.59* 3.45 ± 0.06* -
Pyruvate + DLA 3.86 ± 0.23 17.58 ± 2.21* 4.56 ± 0.51* -
LLA + DLA 3.76 ± 0.21 11.78 ± 0.41 3.14 ± 0.07* -
  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate), DLA or LLA (5 mM + 2.5 mM malate), and LLA+DLA (2.5 mM LLA and 2.5 mM DLA+ 2.5 mM malate).
  3. cCocktail contains 62.5 μM each of malate, glutamate, alpha-ketoglutarate and pyruvate.
  4. State 4: The respiration state without ADP.
  5. State 3: The respiration state with the addition of ADP.
  6. RCR, Respiratory Control Ratio (state 3 rate:state 4 rate).
  7. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  8. *Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test, α < 0.05.