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Table 4 Respiratory parametersa of isolated rat brain and liver mitochondria with pyruvate, D-lactate, L-lactate as substrates in the presence of oxamate, a LDH inhibitor.

From: D-Lactate altered mitochondrial energy production in rat brain and heart but not liver

Substrateb Tissue State 4 State 3 RCI
Pyruvate + OX Brain 3.20 ± 0.57 17.96 ± 2.51 5.74 ± 0.41
  Liver 2.61 ± 0.09 9.98 ± 0.31 3.83 ± 0.05
LLA + OX Brain 3.45 ± 0.12 4.27 ± 0.35 1.23 ± 0.06
  Liver 3.28 ± 0.23 4.19 ± 1.08 1.25 ± 0.23
DLA + OX Brain 3.25 ± 0.15 4.83 ± 1.11 1.47 ± 0.30
  Liver 4.11 ± 0.15 14.34 ± 0.21 3.50 ± 0.12
Pyr +LLA+ OX Brain 3.06 ± 0.42 7.14 ± 1.23 2.32 ± 0.14
  Liver 2.16 ± 0.22 2.73 ± 0.45 1.29 ± 0.25
  1. aRespiratory rates in nmol O2/mg mitochondrial protein per min.
  2. bSubstrate concentrations: pyruvate (10 mM + 2.5 mM malate) and LLA or DLA (5 mM + 2.5 mM malate); OX, oxamate (50 mM).
  3. State 4: The respiration state without ADP.
  4. State 3: The respiration state with the addition of ADP.
  5. RCR: Respiratory Control Ratio (state 3 rate:state 4 rate).
  6. ADP/O ratio: The ratio between the amount of ADP phosphorylated and oxygen consumed.
  7. *Significantly different from the values without substrate. Values were compared using one way ANOVA with LSD as post hoc test. α < 0.05
  8. Note: Refer to Table 1 and 2 for the respiratory parameters without substrates or with cocktail.