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Table 2 Effects of theophylline (50 mg/kg bw) on soleus muscle and heart mRNA content of the different proteolytic systems in rats bearing the Yoshida AH-130 ascites hepatoma

From: Theophylline is able to partially revert cachexia in tumour-bearing rats

   SOLEUS      HEART    
Treatment C(n = 5) T(n = 5) T + TPH(n = 6) ANOVA C(n = 5) T(n = 5) T + TPH(n = 6) ANOVA
     A B     A B
PROTEOLYTIC SYSTEM
Ubiquitin-dependent
Ubiquitin 100 ± 6 204 ± 15 118 ± 18 0,000 0,001 100 ± 5 302 ± 51 177 ± 32 0,001 0,019
Proteasome subunit C2 100 ± 13 168 ± 10 160 ± 40 ns ns 100 ± 3 303 ± 47 191 ± 23 0,000 0,010
Proteasome subunit C8 100 ± 5 111 ± 8 97 ± 25 ns ns 100 ± 3 262 ± 26 164 ± 22 0,000 0,005
E2 100 ± 5 527 ± 129 288 ± 80 0,004 0,063 100 ± 5 224 ± 55 114 ± 19 0,013 0,025
MuRF-1 100 ± 3 171 ± 19 80 ± 26 0,025 0,007 100 ± 3 309 ± 92 177 ± 38 0,015 ns
Atrogin-1 100 ± 8 392 ± 22 362 ± 71 0,002 ns 100 ± 3 161 ± 21 152 ± 21 0,039 ns
Calcium-dependent
m-calpain 100 ± 4 117 ± 23 142 ± 43 ns ns 100 ± 4 307 ± 58 170 ± 18 0,001 0,009
Lysosomal
Cathepsin-B 100 ± 4 111 ± 8 151 ± 34 ns ns 100 ± 2 194 ± 40 111 ± 13 0,010 ns
  1. For further details, see the Material and Methods section. The results are expressed as a percentage of controls (T). All data are mean ± SEM. C = non-tumour-bearing rats, T = tumour-bearing rats, T + TPH = tumour-bearing rats treated with theophylline. Statistical significance of the results by two-way analysis of variance (ANOVA) are indicated by: ns (non-significant differences), A (tumour effect), B (treatment effect).