Table 2 Effects of theophylline (50 mg/kg bw) on soleus muscle and heart mRNA content of the different proteolytic systems in rats bearing the Yoshida AH-130 ascites hepatoma
From: Theophylline is able to partially revert cachexia in tumour-bearing rats
SOLEUS | HEART | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Treatment | C(n = 5) | T(n = 5) | T + TPH(n = 6) | ANOVA | C(n = 5) | T(n = 5) | T + TPH(n = 6) | ANOVA | ||
A | B | A | B | |||||||
PROTEOLYTIC SYSTEM | ||||||||||
Ubiquitin-dependent | ||||||||||
Ubiquitin | 100 ± 6 | 204 ± 15 | 118 ± 18 | 0,000 | 0,001 | 100 ± 5 | 302 ± 51 | 177 ± 32 | 0,001 | 0,019 |
Proteasome subunit C2 | 100 ± 13 | 168 ± 10 | 160 ± 40 | ns | ns | 100 ± 3 | 303 ± 47 | 191 ± 23 | 0,000 | 0,010 |
Proteasome subunit C8 | 100 ± 5 | 111 ± 8 | 97 ± 25 | ns | ns | 100 ± 3 | 262 ± 26 | 164 ± 22 | 0,000 | 0,005 |
E2 | 100 ± 5 | 527 ± 129 | 288 ± 80 | 0,004 | 0,063 | 100 ± 5 | 224 ± 55 | 114 ± 19 | 0,013 | 0,025 |
MuRF-1 | 100 ± 3 | 171 ± 19 | 80 ± 26 | 0,025 | 0,007 | 100 ± 3 | 309 ± 92 | 177 ± 38 | 0,015 | ns |
Atrogin-1 | 100 ± 8 | 392 ± 22 | 362 ± 71 | 0,002 | ns | 100 ± 3 | 161 ± 21 | 152 ± 21 | 0,039 | ns |
Calcium-dependent | ||||||||||
m-calpain | 100 ± 4 | 117 ± 23 | 142 ± 43 | ns | ns | 100 ± 4 | 307 ± 58 | 170 ± 18 | 0,001 | 0,009 |
Lysosomal | ||||||||||
Cathepsin-B | 100 ± 4 | 111 ± 8 | 151 ± 34 | ns | ns | 100 ± 2 | 194 ± 40 | 111 ± 13 | 0,010 | ns |