Figure 3

Effect of ENF on physiologic variables under isocaloric conditions. (A) Cumulative food intake during ZT 12–18, ZT 18–24 and ZT 0–12. C57B/6 mice were fed with a certain amount of chow, which was restricted to 90% of their basal consumption, for 4 consecutive days (10.8 kcal per day). The food was provided at ZT 12 (IC-control) or ZT 18 (ENF-CR) each day. The amount of food intake was obtained by the subtraction of the amount of food remaining at the end of the term from the amount offered at the beginning of each term. (B) Body weight loss during the consecutive 4 days, when treated with either IC-control or ENF-CR. The body weight loss was calculated from the body weight values before (day 0) and after (day 4) treatment. (C) Liver and (D) epididymal fat weights. Tissue weights were measured at ZT 0, 4, 8, 12, 16 and 20 on day 4. Dark periods are indicated by the gray area. All of the values are expressed as the mean +/- SEM (n = 5 or n = 6 per time point). *p < 0.05 for the ENF-CR group compared with the IC-control.