Fig. 2

Internalization of macrophage-derived MVs into adipocytes. a. Micrographs of MVs isolated from culture medium of THP-1 monocytes and polarized THP-1 macrophages examined by TEM. These particles are highlighted by red arrows. Scale bars are all equal to 100 nm. b. Equal amounts of proteins from indicated MVs were analyzed using western blotting for exosome-enriched protein TSG10. Western blot images were analyzed by Bandscan software, and statistical analysis is presented below. c. Internalization of fluorescently labeled M0 MVs into adipocytes was analyzed by confocal microscopy (400×)