Fig. 4

Effect of S. indicus extract and 7 HF on the nuclear translocation of NF-kB p65 in TNF-α-stimulated HUVECs. Briefly, confluent HUVEC monolayers were pretreated with 15 μg/mL S. indicus extract or 3 μM 7 HF for 1 h followed by stimulation with 0.1nM TNF-α for the time points. The cytosolic (Panel a) as well as nuclear (Panel b) extracts were prepared (as described in materials and methods) and analyzed by Western blot analysis with a primary antibody against human NF-kB p65 (3 independent experiments). Upper row shows treatment with TNF-α, middle rows shows treatment with TNF-α + S. indicus extract, and lower row shows TNF-α + 7-HF