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Fig. 5 | Nutrition & Metabolism

Fig. 5

From: A novel anti-inflammatory natural product from Sphaeranthus indicus inhibits expression of VCAM1 and ICAM1, and slows atherosclerosis progression independent of lipid changes

Fig. 5

Effect of S. indicus extract and 7 HF on TNF-α-induced phosphorylation and degradation of IkB-α. Briefly confluent HUVEC monolayers were pretreated with 15 μg/mL S. indicus extract or 3 μM 7 HF for 1 h followed by stimulation with 0.1 nM TNF-α for the time points. The cytosolic extracts were prepared (as described in materials and methods) and then analyzed by Western blot analysis with a primary antibody against human IkB-α (a) phosphorylated IkB-α Κ (b) (3 independent experiments)

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