Fig. 3
LDLR protein is stabilized in S1Pf/f Cre mice livers. a, A schematic depicting the Gau-LDLR fusion protein reporter; b, Huh7 cells were transfected with empty vector, wild-type LDLR or a Gau-LDLR plasmid. Forty-eight hours after transfection, cell lysates were collected and LDLR expression was confirmed by immunoblotting; c and d, Another two sets of cells were subjected to DiI-LDL binding or uptake, respectively, as described in Materials and Methods; e, Relative luciferase (Gaussia/Firefly) counts in livers of S1Pf/f Empty and S1Pf/fCre mice as a measure of total LDLR protein as described in Material and Methods. Here Ad-Empty was used for S1P control mice as Ad-luciferase was administered in both control and S1P KD mice to serve as the normalizing factor for injection. N = 3, each data point is mean +/- SD, * denotes p < 0.05