Fig. 4

Ezetimibe and simvastatin treatment ameliorate hepatic steatosis in 2.5 % HFC-treated zebrafish larvae. a Whole-mount oil red O staining, oil red O staining of frozen liver sections and H&E staining of paraffin liver sections in larvae exposure to control, 2.5 % HFC, 2.5 % HFC + ezetimibe and 2.5 % HFC + simvastatin diets. Clear cytoplasmic lipid droplets were seen in livers of larvae fed with 2.5 % HFC diet. Ezetimibe and simvastatin treatment decreased lipid droplets in 2.5 % HFC-treated zebrafish larvae. b FC, (c) TC and (d) TG levels in livers of larvae fed with control, 2.5 % HFC, 2.5 % HFC + ezetimibe and 2.5 % HFC + simvastatin diets for 10 days were normalized to total protein. Data are expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, by one-way ANOVA