Bacterial metabolites directly modulate farnesoid X receptor activity

Nutrition & Metabolism

Table 2 Sequences of primers used in this study

Gene	Forward	Reverse	Reference
GAPDH	GAAGGTGAAGGTCGGAGT	CATGGGTGGAATCATATTGGAA	[45]
IBABP	TCACTTGGTCCCAGCACTA	CTTGTCACCCACGATCTCT	[45]
OSTα	CTACACCTGGGTGAGCAGAA	AGAGGAATAGGGAGGCGAAC	[46]
FGF19	CACGGGCTCTCCAGCTGCTTCCTGCG	TCCTCCTCGAAAGCACAGTCTTCCTCCG	[47]
SHP	GGCTGGCAGTGCTGATTCAG	TGGGGTGTGGCTGAGTGAAG	[48]
Gapdh	AGGTCGGTGTGAACGGATTTG	TGTAGACCATGTAGTTGAGGTCA	[22]
Fxr	TCCAGGGTTTCAGACACTGG	GCCGAACGAAGAAACATGG	[27]
Ibabp	CAGGAGACGTGATTGAAAGGG	GCCCCCAGAGTAAGACTGGG	[27]
Ostα	TGTTCCAGGTGCTTGTCATCC	CCACTGTTAGCCAAGATGGAGAA	[27]
Fgf15	ACGTCCTTGATGGCAATCG	GAGGACCAAAACGAACGAAAT T	[27]
Cyp7a1	AGCAACTAAACAACCTGCCAGTACTA	GTCCGGATATTCAAGGATGCA	[27]
Cyp7b1	TAGCCCTCTTTCCTCCACTCATA	GAACCGATCGAACCTAAATTCCT	[27]
Cyp8b1	GGCTGGCTTCCTGAGCTTATT	ACTTCCTGAACAGCTCATCGG	[27]
Shp	CGATCCTCTTCAACCCAGATG	AGGGCTCCAAGACTTCACACA	[27]
Bsep	CTGCCAAGGATGCTAATGCA	CGATGGCTACCCTTTGCTTCT	[27]
Ntcp	ATGACCACCTGCTCCAGCTT	GCCTTTGTAGGGCACCTTGT	[27]
Ibat	ACCACTTGCTCCACACTGCTT	CGTTCCTGAGTCAACCCACAT	[27]

Genes in capitals indicate human genes, and those in lower case are mouse genes. Gapdh, Ibabp and Ostα were analyzed using the following conditions: 95 °C 2 min, 40 cycles of 95 °C for 10 s, and 60 °C for 30 s. Fgf19 was analyzed using the following conditions: 95 °C 15 min, 40 cycles of 95 °C for 15 s, and 62 °C for 30 s. All mouse genes were analyzed using the following conditions: 95 °C 2 min, 40 cycles of 95 °C for 15 s, and 60 °C for 60 s

ISSN: 1743-7075