Fig. 2

Evolution of hepatic complications. a Severity of hepatic complications was assessed by eosin/hematoxylin coloration and Oil-Red O for steatosis, Masson’s Trichrome for fibrosis, macrophages infiltration and oxidative stress by dihydroethidine (DHE) at the beginning (M0), after 2 (M2) and 8 (M8) months of normal diet (ND), normal diet + fructose (HF), high fat diet (HFD) and HFD + fructose (HFHF). The score of steatosis is noted under the picture (0, 1, 2, or 3). Results for 5 different experiments are shown. Bar scale = 100 μm. b-c Quantification of hepatic triglycerides (b) and glycogen (c) in frozen-liver tissue, at the beginning (M0), after 2 (M2) and 8 (M8) months of normal diet (ND), normal diet + fructose (HF), high fat diet (HFD) and HFD + fructose (HFHF). Results are shown as mean ± SEM of 5 different experiments. * Significant results versus age-matched ND-rats and # between two groups