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Table 2 Gene expression analyses by qRT-PCR for diet and genotype comparisons

From: Short chain acyl-CoA dehydrogenase deficiency and short-term high-fat diet perturb mitochondrial energy metabolism and transcriptional control of lipid-handling in liver

  Diet comparisons   Genotype comparisons
Gene Symbol Microarray qRT-PCR Gene Symbol Microarray qRT-PCR
FC p-value FC p-value FC p-value FC p-value
Acads−/− HF vs. LF HF Acads−/− vs. Acads+/+
Acacb ND ND −2.86 0.001 Acacb 4.02 0.230 1.14 0.652
G6pc −3.20 0.000 −2.94 0.000 G6pc ND ND −1.07 0.606
Nr0b2 (SHP-1) −1.87 0.000 1.40 0.063 Nr0b2 ND ND 1.33 0.253
Nr1h3 (LXR) ND ND 1.15 0.234 Nr1h3 ND ND −1.08 0.512
Nr1h4 (FXR) ND ND −1.10 0.619 Nr1h4 ND ND 1.22 0.308
Nr1i2 (PXR) ND ND −1.31 0.081 Nr1i2 ND ND −1.38 0.024
Nr1i3 (CAR) 2.40 0.000 4.23 0.001 Nr1i3 ND ND 1.43 0.161
Pklr −5.28 0.000 −3.93 0.000 Pklr ND ND 1.18 0.372
Rxra 2.28 0.000 1.56 0.004 Rxra ND ND 1.00 0.981
Srebf1 −2.03 0.000 −1.61 0.095 Srebf1 ND ND −1.04 0.829
Sult1a1(Phase II DME) 2.55 0.000 3.28 0.000 Sult1a1 ND ND 1.40 0.666
Acads+/+ HF vs. LF   LF Acads−/− vs. Acads+/+
Acacb −5.13 0.000 −2.99 0.003 Acacb ND ND 1.09 0.704
G6pc ND ND −1.67 0.001 G6pc ND ND 1.64 0.000
Nr0b2 (SHP-1) ND ND −1.12 0.719 Nr0b2 ND ND −1.18 .551
Nr1h3 (LXR) ND ND 1.07 0.757 Nr1h3 ND ND −1.17 0.457
Nr1h4 (FXR) ND ND 1.16 0.568 Nr1h4 ND ND 1.56 0.125
Nr1i2 (PXR) ND ND 1.32 0.197 Nr1i2 ND ND 1.25 0.311
Nr1i3 (CAR) 1.87 0.000 2.60 0.002 Nr1i3 ND ND −1.14 0.688
Pklr −3.56 0.000 −3.65 0.000 Pklr ND ND 1.27 0.188
Rxra ND ND 1.31 0.034 Rxra ND ND −1.19 0.147
Srebf1 −1.71 0.009 −1.56 0.070 Srebf1 ND ND −1.01 0.974
Sult1a1(Phase II DME) ND ND 1.67 0.009 Sult1a1 ND ND −1.41 0.046
  1. Representative genes were selected to validate the microarray results in liver. Overall, we observed concordant changes in the array and the qPCR. With the diet comparisons alone, 12 PCR assays reached statistical significance in a two-tailed Student’s t-test performed on ΔCt values
  2. ND, not detected