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Table 2 Gene expression analyses by qRT-PCR for diet and genotype comparisons

From: Short chain acyl-CoA dehydrogenase deficiency and short-term high-fat diet perturb mitochondrial energy metabolism and transcriptional control of lipid-handling in liver

 

Diet comparisons

 

Genotype comparisons

Gene Symbol

Microarray

qRT-PCR

Gene Symbol

Microarray

qRT-PCR

FC

p-value

FC

p-value

FC

p-value

FC

p-value

Acads−/− HF vs. LF

HF Acads−/− vs. Acads+/+

Acacb

ND

ND

−2.86

0.001

Acacb

4.02

0.230

1.14

0.652

G6pc

−3.20

0.000

−2.94

0.000

G6pc

ND

ND

−1.07

0.606

Nr0b2 (SHP-1)

−1.87

0.000

1.40

0.063

Nr0b2

ND

ND

1.33

0.253

Nr1h3 (LXR)

ND

ND

1.15

0.234

Nr1h3

ND

ND

−1.08

0.512

Nr1h4 (FXR)

ND

ND

−1.10

0.619

Nr1h4

ND

ND

1.22

0.308

Nr1i2 (PXR)

ND

ND

−1.31

0.081

Nr1i2

ND

ND

−1.38

0.024

Nr1i3 (CAR)

2.40

0.000

4.23

0.001

Nr1i3

ND

ND

1.43

0.161

Pklr

−5.28

0.000

−3.93

0.000

Pklr

ND

ND

1.18

0.372

Rxra

2.28

0.000

1.56

0.004

Rxra

ND

ND

1.00

0.981

Srebf1

−2.03

0.000

−1.61

0.095

Srebf1

ND

ND

−1.04

0.829

Sult1a1(Phase II DME)

2.55

0.000

3.28

0.000

Sult1a1

ND

ND

1.40

0.666

Acads+/+ HF vs. LF

 

LF Acads−/− vs. Acads+/+

Acacb

−5.13

0.000

−2.99

0.003

Acacb

ND

ND

1.09

0.704

G6pc

ND

ND

−1.67

0.001

G6pc

ND

ND

1.64

0.000

Nr0b2 (SHP-1)

ND

ND

−1.12

0.719

Nr0b2

ND

ND

−1.18

.551

Nr1h3 (LXR)

ND

ND

1.07

0.757

Nr1h3

ND

ND

−1.17

0.457

Nr1h4 (FXR)

ND

ND

1.16

0.568

Nr1h4

ND

ND

1.56

0.125

Nr1i2 (PXR)

ND

ND

1.32

0.197

Nr1i2

ND

ND

1.25

0.311

Nr1i3 (CAR)

1.87

0.000

2.60

0.002

Nr1i3

ND

ND

−1.14

0.688

Pklr

−3.56

0.000

−3.65

0.000

Pklr

ND

ND

1.27

0.188

Rxra

ND

ND

1.31

0.034

Rxra

ND

ND

−1.19

0.147

Srebf1

−1.71

0.009

−1.56

0.070

Srebf1

ND

ND

−1.01

0.974

Sult1a1(Phase II DME)

ND

ND

1.67

0.009

Sult1a1

ND

ND

−1.41

0.046

  1. Representative genes were selected to validate the microarray results in liver. Overall, we observed concordant changes in the array and the qPCR. With the diet comparisons alone, 12 PCR assays reached statistical significance in a two-tailed Student’s t-test performed on ΔCt values
  2. ND, not detected
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Nutrition & Metabolism

ISSN: 1743-7075

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