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Table 1 Protective effect of unsaturated NEFAs against PA-induced toxicity and relative lipid droplet area linked with the correlation coefficient for each group

From: The role of lipid droplet formation in the protection of unsaturated fatty acids against palmitic acid induced lipotoxicity to rat insulin-producing cells

NEFA Half-maximal protective concentration (μM) Lipid droplet area after incubation with 100 μM NEFA (% of cell area) Correlation coefficient for each group P value for each group
C18:1 32.8 ± 2.2 (6) 7.3 ± 1.3 (5) C18-FFA: +0.52 C18-FFA: 0.37
C18:2 11.4 ± 1.2 (5) 15.1 ± 2.8 (5)
α-C18:3 24.0 ± 1.8 (4) 2.4 ± 0.6 (5)
γ-C18:3 27.2 ± 1.6 (4) 2.6 ± 0.5 (5)
C18:4 > 100.0 (5) 1.9 ± 0.3 (5)
C20:1 17.2 ± 1.6 (6) 26.5 ± 4.9 (5) C20-FFA: −0.63 C20-FFA: 0.25
C20:2 6.0 ± 0.6 (4) 19.2 ± 2.9 (5)
C20:3 10.4 ± 1.2 (4) 8.1 ± 1.3 (5)
C20:4 8.6 ± 0.6 (4) 2.7 ± 0.6 (5)
C20:5 5.4 ± 0.6 (4) 3.9 ± 1.0 (5)
C22:1 88.6 ± 22.8 (6) 19.7 ± 4.1 (5) C22-FFA: −0.89 C22-FFA: 0.11
C22:2 42.0 ± 6.8 (4) 18.9 ± 2.7 (5)
C22:4 8.6 ± 1.4 (5) 12.1 ± 2.5 (5)
C22:6 10.8 ± 3.2 (5) 11.8 ± 2.4 (5)
Total value    0.04 0.89
  1. The protective concentration of the unsaturated NEFAs was determined in co-incubation experiments for 24 h with 200 μM PA resulting in a 50 % cell viability measured by MTT assay. The protective effects were calculated by nonlinear regression analyses. Lipid droplet and MTT data are means ± SEM with the number of independent experiments in parenthesis. Correlation coefficients and P values were determined by Pearson correlation