|
NEFA
|
Half-maximal protective concentration (μM)
|
Lipid droplet area after incubation with 100 μM NEFA (% of cell area)
|
Correlation coefficient for each group
|
P value for each group
|
|---|
|
C18:1
|
32.8 ± 2.2 (6)
|
7.3 ± 1.3 (5)
|
C18-FFA: +0.52
|
C18-FFA: 0.37
|
|
C18:2
|
11.4 ± 1.2 (5)
|
15.1 ± 2.8 (5)
|
|
α-C18:3
|
24.0 ± 1.8 (4)
|
2.4 ± 0.6 (5)
|
|
γ-C18:3
|
27.2 ± 1.6 (4)
|
2.6 ± 0.5 (5)
|
|
C18:4
|
> 100.0 (5)
|
1.9 ± 0.3 (5)
|
|
C20:1
|
17.2 ± 1.6 (6)
|
26.5 ± 4.9 (5)
|
C20-FFA: −0.63
|
C20-FFA: 0.25
|
|
C20:2
|
6.0 ± 0.6 (4)
|
19.2 ± 2.9 (5)
|
|
C20:3
|
10.4 ± 1.2 (4)
|
8.1 ± 1.3 (5)
|
|
C20:4
|
8.6 ± 0.6 (4)
|
2.7 ± 0.6 (5)
|
|
C20:5
|
5.4 ± 0.6 (4)
|
3.9 ± 1.0 (5)
|
|
C22:1
|
88.6 ± 22.8 (6)
|
19.7 ± 4.1 (5)
|
C22-FFA: −0.89
|
C22-FFA: 0.11
|
|
C22:2
|
42.0 ± 6.8 (4)
|
18.9 ± 2.7 (5)
|
|
C22:4
|
8.6 ± 1.4 (5)
|
12.1 ± 2.5 (5)
|
|
C22:6
|
10.8 ± 3.2 (5)
|
11.8 ± 2.4 (5)
|
|
Total value
| | |
0.04
|
0.89
|
- The protective concentration of the unsaturated NEFAs was determined in co-incubation experiments for 24 h with 200 μM PA resulting in a 50 % cell viability measured by MTT assay. The protective effects were calculated by nonlinear regression analyses. Lipid droplet and MTT data are means ± SEM with the number of independent experiments in parenthesis. Correlation coefficients and P values were determined by Pearson correlation
