Table 2 List of primer/probe sets
From: Evaluation of markers of beige adipocytes in white adipose tissue of the mouse
Gene | Forward primer | Reverse primer | Probe |
|---|---|---|---|
UCP1 | GCATTCAGAGGCAAATCAGC | GCCACACCTCCAGTCATTAAG | 56-FAM/ACTGAGTCG/Zen/TAGA GGCCAATCCTGA/3IABkFQ |
CIDEA | CATACATGCTCCGAGTACTGG | CATCCCACAGCCTATAACAGAG | 56-FAM/TAACCAGGG/Zen/CACA GCTACAGAGG/3IABkFQ |
Cox8b | AGCCAAAACTCCCACTTCC | TCTCAGGGATGTGCAACTTC | 56-FAM/AGATCCCCA/Zen/C AGCCTGCTCC/3IABkFQ |
Serpina3k | GCCAAAGTCAATAACCCCAAG | CTTTGCAACAGCCAATCAGAG | 56-FAM/AGCCCTGGA/Zen/C AGAATCATGAGAAC/3IABkFQ |
Wdnm1-like | GCCAGAGGAACAATGTGTCAG | GTAATCTCCATACATGGCCTCC | 56-FAM/TGGTCACAG/Zen/AGCT CCAAACAGACAC/3IABkFQ |
ASC1 | TCTTCATTTCCATCCCACTGG | ATGACCCACGAAAAGTAGCC | 56-FAM/ATGTCGCCT/Zen/ACTT CACTGCCATGT/3IABkFQ |
FGF21 | CAAATCCTGGGTGTCAAAGC | CATGGGCTTCAGACTGGTAC | 56-FAM/CTCCATCTG/Zen/GCTG TTGGCAAAGAAA/3IABkFQ |
P2RX5 | TGATAGTTAATGGCAAGGCGG | TTGTCTCGGTAAAACTCGCTC | 56-FAM/CCAGGTCAC/Zen/A GAAGAAAGCCCCA/3IABkFQ |
PAT2 | AGCCACCCCTCTCAATCT | TGCCTTTGACCAGATGAACC | 56-FAM/CCAGGATCC/Zen/C AGTCCAGCGAATG/3IABkFQ/ |
CITED1 | ATTTATCGGACTTCTGCCCAG | TTGCGATCCTTCACTCCAAG | 56-FAM/AGGCCTCGA/Zen/CATA GTTGGCATTTCA/3IABkFQ |
CAR4 | CAGAGCACAGTATTGATGGGAG | CTTGTTCACCTTGTCTCCTACC | 56-FAM/TTGTCCTTC/Zen/GAGT CCTCCTTGCTAGA/3IABkFQ |
TBX1 | TGGGACGAGTTCAATCAGC | TGTCATCTACGGGCACAAAG | 56-FAM/TCCCCACGT/Zen/TCCA AGTGAAGCTTTT/3IABkFQ |
CD137 | CCTGTGATAACTGTCAGCCTG | TCTTGAACCTGAAATAGCCTGC | 56-FAM/TGCCCTCCA/Zen/AGTA CCTTCTCCAG/3IABkFQ |
TMEM26 | GCACCATCACTAGAGACCAAC | ACAAGAATGCCAGAGACCAG | 56-FAM/CGCCGTCCC/Zen/C ACAAACATCAGA/3IABkFQ |
PDGFR-α | GTTGCCTTACGACTCCAGATG | TCACAGCCACCTTCATTACAG | 56-FAM/TCCCAAAAT/Zen/CCGA CCAAGCACGA/3IABkFQ |