Fig. 3

HDL (V) is preβ1-HDL. To prove that HDL-(V) is preβ1-HDL, we isolated different HDL fractions by ultracentrifugation. a Isolation of non-HDL and HDL particles using ultracentrifugation. b Each fraction was subjected to our new PAGE system. The red arrowhead indicates HDL (V). c Each fraction was subjected to our new two-dimensional gel electrophoresis and then immunoblotted with anti-apoA-I. HDL-(V) exhibited an electrophoretic pattern identical to that preβ1-HDL