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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane

Fig. 3

Protein immuno-detection in intestinal CLD by Western-blot and cytometry. (a) Western-blot analysis in the total homogenate I or in the droplets D1, D2 or D3 showing discrete expression of SR-BI, CD36, ABCG5, G8 and A1, caveolin (cav) 1 and 2, and the I- and L-FABP. Equivalent protein amounts were loaded for each sample and proteins molecular weights are noted in kDa. (b, c, d) Immuno-detection by flow cytometry of NPC1L1, SR-BI and NPC1L1 plus SR-BI, respectively, in D1 droplets already fixed with PFA. Bars: 10 μM

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