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Table 1 Principal characteristics of intestinal CLD, D1, D2 and D3

From: Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane

CLD

Centrifugation

d (g/ml)

Size (mean diameter ± SD)

Protein content in % of total in I

D1

20,000 g 30 min

1

793 ± 409 nm (n = 40)

6 %

D2

100,000 g 2 h

1

63 ± 42 nm (n = 39)

1 to 3 %

D3

120,000 g 14 h,

1.21

13 ± 4 nm (n = 25)

0.5 to 1 %

  1. Is noted the speed of the last centrifugation performed to overload the droplets, the density of the solution used, the size of the droplet measured by TEM and the percent of protein content in the droplets compared to the total content in the initial homogenate