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Table 2 MGAT enzymatic assays using 100 μg of proteins from either microsomal/basolateral membranes (Mi/Ba) or BBM

From: Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane

  Mic/ Ba BBM
Lipids nmol/mg nmol/mg
DG 16–16 11,23 4,22
DG 16–18 35,80 11,83
DG 18–18 0,00 8,69
DG18–20 0,00 0,00
Total DG 47,03 24,73
Chol-C16 14,33 0,00
Chol-C18 29,21 0,00
Total CE 43,54 0,00
C53-TG (16/16/18) 60,54 1,51
C55-TG (16/18/18) 118,91 4,25
C57-TG (18/18/18) 104,93 16,67
C59- TG (18/18/20) 13,58 2,05
Total TG 311,22 24,48
Total DG + TG + CE 401,79 49,21
  1. The assay measured the production of diacylglycerols (DG), cholesterol ester (CE) and triglycerides (TG) in nmoles/mg of proteins, by the membranes incubated for 90 min at 22 °C in the presence of 50 μM of mono-oleoylglycerol and 50 μM of oleoyl-CoA