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Table 2 MGAT enzymatic assays using 100 μg of proteins from either microsomal/basolateral membranes (Mi/Ba) or BBM

From: Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane

 

Mic/ Ba

BBM

Lipids

nmol/mg

nmol/mg

DG 16–16

11,23

4,22

DG 16–18

35,80

11,83

DG 18–18

0,00

8,69

DG18–20

0,00

0,00

Total DG

47,03

24,73

Chol-C16

14,33

0,00

Chol-C18

29,21

0,00

Total CE

43,54

0,00

C53-TG (16/16/18)

60,54

1,51

C55-TG (16/18/18)

118,91

4,25

C57-TG (18/18/18)

104,93

16,67

C59- TG (18/18/20)

13,58

2,05

Total TG

311,22

24,48

Total DG + TG + CE

401,79

49,21

  1. The assay measured the production of diacylglycerols (DG), cholesterol ester (CE) and triglycerides (TG) in nmoles/mg of proteins, by the membranes incubated for 90 min at 22 °C in the presence of 50 μM of mono-oleoylglycerol and 50 μM of oleoyl-CoA
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Nutrition & Metabolism

ISSN: 1743-7075

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