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Table 1 Information about the primers used for determination of 16S rRNA genes by qPCR

From: Dietary green-plant thylakoids decrease gastric emptying and gut transit, promote changes in the gut microbial flora, but does not cause steatorrhea

Target bacterial group Primer Sequence (5′-3′) Amplicon size (bp) Annealing and elongation time (s) Reference
Total bacteria F-tot GCA GGC CTA ACA CAT GCA AGT C 292 15 [39]
R-tot CTG CTG CCT CCC GTA GGA GT
Akkermansia muciniphilia-like bacteria AM1-F CAG CAC GTG AAG GTG GGG AC 327 20 [40]
AM2-R CCT TGC GGT TGG CTT CAG AT
Bacteroides fragilis group Bfra-F ATA GCC TTT CGA AAG RAA GAT 495 30 [41]
Bfra-R CCA GTA TCA ACT GCA ATT TTA
Clostridium coccoides group CcocF AAA TGA CGG TAC CTG ACT AA 440 20 [41]
CcocR CTT TGA GTT TCA TTC TTG CGA A
Clostridium leptum subgroup CleptF GCA CAA GCA GTG GAG T 239 20 [42]
CleptR3 CTT CCT CCG TTT TGT CAA
Enterobacteriaecae Eco1457-F CAT TGA CGT TAC CCG CAG AAG AAG C 195 10 [43]
Eco1652-R CTC TAC GAG ACT CAA GCT TGC
Lactobacillus Lact-F AGC AGT AGG GAA TCT TCC A 341 20 [44]
Lact-R CAC CGC TAC ACA TGG AG [45]