Target bacterial group | Primer | Sequence (5′-3′) | Amplicon size (bp) | Annealing and elongation time (s) | Reference |
---|---|---|---|---|---|
Total bacteria | F-tot | GCA GGC CTA ACA CAT GCA AGT C | 292 | 15 | [39] |
R-tot | CTG CTG CCT CCC GTA GGA GT | ||||
Akkermansia muciniphilia-like bacteria | AM1-F | CAG CAC GTG AAG GTG GGG AC | 327 | 20 | [40] |
AM2-R | CCT TGC GGT TGG CTT CAG AT | ||||
Bacteroides fragilis group | Bfra-F | ATA GCC TTT CGA AAG RAA GAT | 495 | 30 | [41] |
Bfra-R | CCA GTA TCA ACT GCA ATT TTA | ||||
Clostridium coccoides group | CcocF | AAA TGA CGG TAC CTG ACT AA | 440 | 20 | [41] |
CcocR | CTT TGA GTT TCA TTC TTG CGA A | ||||
Clostridium leptum subgroup | CleptF | GCA CAA GCA GTG GAG T | 239 | 20 | [42] |
CleptR3 | CTT CCT CCG TTT TGT CAA | ||||
Enterobacteriaecae | Eco1457-F | CAT TGA CGT TAC CCG CAG AAG AAG C | 195 | 10 | [43] |
Eco1652-R | CTC TAC GAG ACT CAA GCT TGC | ||||
Lactobacillus | Lact-F | AGC AGT AGG GAA TCT TCC A | 341 | 20 | [44] |
Lact-R | CAC CGC TAC ACA TGG AG | [45] |