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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Metabolic response of glioblastoma cells associated with glucose withdrawal and pyruvate substitution as revealed by GC-MS

Fig. 3

Metabolic pathway of U87 cells profiling data. A combined metabolic pathway is presented, including glycolysis, the pentose-phosphate pathway, the TCA cycle and branched chain amino acid degradation. Intracellular metabolite abundances (Fig. 2) are shown as bar diagrams depicting the relative abundance which is the: ratio between the abundance of a metabolite (the peak area) normalized to total protein (μg) compared to the abundance at 25 mM glucose (normalized to protein) set as 1. Direct reactions without intermediates are presented as straight lines and reactions involving several steps are presented as dotted lines. Metabolites which were not determined are labeled in Grey. All experiments have been conducted in 6-tuplicate and statistical significance was determined by Student’s t-test with: *: p < 0.05; **: p < 0.005; ***: p < 0.0005. An extended version of the figure including extracellular metabolite concentrations is available in the (Additional file 2: Figure S2). Abbreviations: GA3P: glyceraldehyde-3-phosphate; DHAP: dihydroxyacetone phosphate; Glu: glutamate; aKG: α-ketoglutarate

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