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Table 2 The primers used for quantitative PCR

From: The effects on weight loss and gene expression in adipose and hepatic tissues of very-low carbohydrate and low-fat isoenergetic diets in diet-induced obese mice

Gene

Forward primer (5' to 3')

Reverse primer (5' to 3')

36B4

GGCCCTGCACTCTCGCTTTC

TGCCAGGACGCGCTTGT

Adiponectin

AAGAAGGACAAGGCCGTTCTCTT

GCTATGGGTAGTTGCAGTCAGTT

Arg-1

CTCCAAGCCAAAGTCCTTAGAG

AGGAGCTGTCATTAGGGACATC

BDH

AGTTTGGGGTCGAGGCTTTC

TGGTGGCCGCTATGAAGTTG

CD36

AATGGCACAGACGCAGCCT

GGTTGTCTGGATTCTGGA

CD68antigen

TGACCTGCTCTCTCTAAGGCTACA

TCACGGTTGCAAGAGAAACATG

CD137

CGTGCAGAACTCCTGTGATAAC

GTCCACCTATGCTGGAGAAGG

D2

GCACGTCTCCAATCCTGAAT

TGAACCAAAGTTGACCACCA

Elovl6

TTCCGAGTCTCCCGGAAGT

ACAGCCCATCAGCATCTGAGT

F4/80

TGACAACCAGACGGCTTGTG

GCAGGCGAGGAAAAGATAGTGT

FAS

GCTGCGGAAACTTCAGGAAAT

AGAGACGTGTCACTCCTGGACTT

FGF21

ATGGAATGGATGAGATCTAGAGTTGG

TCTTGGTCGTCATCTGTGTAGAGG

GRP120

GCATAGGAGAAATCTCATGG

GAGTTGGCAAACGTGAAGGC

HADH

ACTACATCAAAATGGGCTCTCAG

AGCAGAAATGGAATGCGGACC

HMGCS2

ATCAACTCCCTGTGCCTGAC

GCAATGTCACCACAGACCAC

Leptin

GACACCAAAACCCTCAT

CAGAGTCTGGTCCATCT

MCAD

GATCGCAATGGGTGCTTTTGATAGAA

AGCTGATTGGCAATGTCTCCAGCAAA

MCP-1

CTTCTGGGCCTGCTGTTCA

CCAGCCTACTCATTGGGATCA

Mincle

ACCAAATCGCCTGCATCC

CACTTGGGAGTTTTTGAAGCATC

MR

CCACAGCATTGAGGAGTTTG

ACAGCTCATCATTTGGCTCA

NOS2

CAGCTGGGCTGTACAAACCTT

CATTGGAAGTGAAGCGTTTCG

PGC1α

AAGTGTGGAACTCTCTGGAACTG

GGGTTATCTTGGTTGGCTTTATG

PPARα

CCTCAGGGTACCACTACGGAGT

GCCGAATAGTTCGCCGAA

PPARγ1

GAGTGTGACGACAAGATTTG

GGTGGGCCAGAATGGCATCT

PPARγ2

TCTGGGAGATTCTCCTGTTGA

GGTGGGCCAGAATGGCATCT

SCD1

CCCCTGCGGATCTTCCTTAT

AGGGTCGGCGTGTGTTTCT

SREBP-1c

GGAGCCATGGATTGCACATT

CCTGTCTCACCCCCAGCATA

TBX1

GGCAGGCAGACGAATGTTC

TTGTCATCTACGGGCACAAAG

TMEM26

ACCCTGTCATCCCACAGAG

TGTTTGGTGGAGTCCTAAGGTC

UCP1

GGCCCTTGTAAACAACAAAATAC

GGCAACAAGAGCTGACAGTAAAT

UCP2

ACCAAGGGCTCAGAGCATGCA

TGGCTTTCAGGAGAGTATCTTTG

  1. 36B4 acidic ribosomal phosphoprotein P0, Arg arginase, BDH 3-hydroxybutyrate dehydrogenase, CD36 fatty acid translocase, D2 type 2 iodothyronine deiodinase, Elovl6 elongation of very long-chain fatty acid 6, FAS fatty acid synthase, FGF21 fibroblast growth factor 21, GPR G-protein-coupled receptor, HADH hydroxyacyl-coenzyme A dehydrogenase, HMGCS2 3-hydroxy-3-methylglutaryl-coenzyme A synthase 2, MCAD medium-chain acyl-CoA dehydrogenase, MCP monocyte chemoattractant protein, Mincle the macrophage inducible C-type lectin, MR mannose receptor, NOS nitric oxide synthase, PGC peroxisome proliferator-activated receptor γ coactivator, PPAR peroxisome proliferator-activated receptor, SCD1 stearoyl-CoA desaturase 1, SREBP sterol regulatory element-binding protein, TBX1 T-box 1 transcription factor C, TMEM26 transmembrane protein 26, UCP uncoupling protein
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Nutrition & Metabolism

ISSN: 1743-7075

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