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Fig. 1 | Nutrition & Metabolism

Fig. 1

From: Long-term consumption of an obesogenic high fat diet prior to ischemia-reperfusion mediates cardioprotection via Epac1-dependent signaling

Fig. 1

Feeding regiment and experimental protocol. a Wild type (wt), Epac1−/− and Epac2−/− mice were divided into 6 groups exposed to either a normal chow diet (ND) or a high fat diet (HFD) for 33 ± 1 weeks. One intraperitoneal glucose tolerance test (ipGTT) was performed before start of the feeding experiment (baseline) and another 28 weeks into the feeding protocol. b At the end of the feeding experiment, the hearts were excised and mounted in a Langendorff perfusion system. Hearts were stabilized for 20min, before being subjected to 30min global ischemia (GI) and 60min of reperfusion (Rep)

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