Fig. 6

The effects of deficiency and excess of dietary salts on fat deposition in WT and klf mutant worms. a Worms were fed on normal NGM media or media with various salt concentrations (Ca²⁺, Mg²⁺, or K⁺-free, and 10x of each salt). Oil red O staining was used to measure fat mass in WT and mutant strains. Worms were observed under Olympus U-Tr0.63Xc optics attached to Axioplan Zeiss microscope and photographed using a digital camera Prog Res CF scan (magnification: 200X). Representative images were shown. b The quantitation of fat mass based on Oil-Red staining intensity. Multiple representative images from each treatment were chosen for image quantitation as described in the “Methods”, and the means with standard errors were presented in the chart. p < 0.05 when comparing the fat mass in mutants over WT worms grown on NGM, 0x (Ca²⁺, Mg²⁺, or K⁺-free media), and 10x salt media under each salt condition. Since the WT and klf mutant worms on NGM are identical among three salt conditions, there are 21 bars in panel B instead of 27 corresponding to the number of images in panel A