Table 2 Primer sequences
Gene name | NCBI Reference number | Forward primer | Reverse primer |
|---|---|---|---|
Genes of interest: | |||
  Acc1 | NM_133360.2 | tggtgcagaggtaccgaagtg | cgtagtggccgttctgaaact |
  Atgl | NM_025802.3 | atccgcttgttggagtggct | ctcttggccctcatcaccag |
  Cd36 | NM_001159558.1 | attgcgacatgattaatggcac | gatagacctgcaaatgtcagaggaa |
  Cidea | NM_007702.2 | aggccgtgttaaggaatctg | cccagtactcggagcatgta |
  Cpt1α | NM_013495.2 | gcccatgttgtacagcttcc | tcatcagtggcctcacagac |
  Fabp4 | NM_024406.2 | tcataaccctagatggcggg | ttccaccaccagcttgtcac |
  Fas | NM_007988.3 | gctgggctctatggattaccc | ggtccattgtgtgtgcctgc |
  Fatp1 | NM_011977.3 | attgtggtgcacagcaggtacta | tggtagagtggcaggcagtca |
  Glut4 | NM_009204.2 | cggatgctatgggtccttacg | aacgtccggcctctggttt |
  Gpat | NM_008149.3 | tgtccacaacttcagcggtc | cagcgtacacggcaacgtt |
  HkII | NM_013820.3 | aagacgataaggacggaattcaga | gagcgcgtggacacaatct |
  Hsl | NM_010719.5 | cctgcttggttcaactggaga | tcactccataggctgctgcc |
  Lpl | NM_008509.2 | cacttgtcatctcattcctggatt | ccgttaccgtccatccatg |
  Pdk4 | NM_013743.2 | aagagctggtatatccagagcctg | ttgaccagcgtgtctacaaactc |
  Pparα | NM_011144.6 | agtgccctgaacatcgagtgt | ttcgccgaaagaagccctta |
  Scd1 | NM_009127.4 | cacctgcctcttcgggatttt | gcagccgtgccttgtaagttc |
  Srebp1c | NM_011480.3 | ccggctattccgtgaacatc | caagggcatctgagaactccc |
  Ucp1 |  | assay ID qMmuCID0005832 |  |
  Ucp3 | NM_009464.3 | aacgctcccctaggcaggta | gcagaaaggagggcacaaatc |
Reference genes: | |||
  Canx | NM_007597.3 | agagctcagcctggatcaattc | ttgtagtcctctccacacttatctgg |
  Rpl19 | NM_009078.2 | ttgcctctagtgtcctccgc | cttcctgatctgctgacggg |
  Rps29 | NM_009093.2 | agtcacccacggaagttcgg | gtccaacttaatgaagcctatgtcctt |
  18SrRNA | NR_003278.1 | cgattccgtgggtggtggtg | catgccagagtctcgttcgttatc |
DNA primers | |||
  Nd1 | NC_005089.1 | accaatacgccctttaacaac | aatgggtgtggtattggtagg |
  Lpl | NM_008509 | tcctgatgacgctgattttg | atgtcaacatgccctactgg |