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Table 5 PRP binding to hydrolyzable vs. condensed tannins

From: Salivary proline-rich protein may reduce tannin-iron chelation: a systematic narrative review

Reference Method Tannin type Bond stability Binding affinity
[66] Competitive binding assay 5GG, gallic acid, EGC nd Hydrolyzable tannin > condensed tannin
[62] Competitive binding assay Quebracho and tannic acid Hydrolyzable: 20% greater dissolution of bonds in gastric and enteric digestion Hydrolyzable tannin > condensed tannin
[48] NMR B2, PGG, TGG, PAC monomer Hydrogen bonds associated with hydrolyzable tannins B2 > PGG > TGG>
[47] NMR, DLS EgCG, EGC, PGG Hydrogen bonds associated with hydrolyzable tannins Hydrolyzable tannin > condensed tannin
[63] SDS PAGE Wine or tannic acid Condensed tannins associated with hydrophobic bonds nd
[61] HPLC Hydrolyzable vs. condensed wine extracts nd Increased precipitation of condensed tannin at pH 7.5 c/t hydrolyzable tannin
  1. NMR Nuclear magnetic resonance imaging, DLS dynamic light scattering, SDS-PAGE sodium dodecyl polyacrylamide gel electrophoresis, HPLC high performance liquid chromatography. B2: proanthocyanidin B2, PGG pentagalloylglucose, TGG tetragalloylglucose, PAC proanthocyanidin, EgCG epigallocatechin gallate, ECG epigallocatechin, PRP proline rich protein. nd = not determined