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Fig. 4 | Nutrition & Metabolism

Fig. 4

From: Positive association between musclin and insulin resistance in obesity: evidence of a human study and an animal experiment

Fig. 4

Musclin expression and its circulating musclin levels increased in HFD rats. a: Real-time PCR analysis of musclin expression in gastrocnemius muscles. b: Western blot analysis of musclin protein expression in gastrocnemius muscles and ratio of musclin to β-actin determined by quantitative analysis. c: Radioimmunoassay analysis of musclin content in gastrocnemius muscles. d: Radioimmunoassay analysis of plasma musclin levels. ND: normal diet; HFD: high-fat diet. Values are the mean ± standard error of the mean; (n = 8); *P < 0.05 vs ND

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