Fig. 1

Plasma insulin and glucose concentrations and food consumption during feeding amino acid-deprived diets in rats. Three animal experiments were performed by feeding a control diet with 20% amino acids (20AA) or a diet deprived of single amino acid for 8 h; Asp, Lys, Pro, Ser, Thr, or Val (A, D, G), Gly, Leu, Met, Arg, or Tyr (B, E, H), and Ala, Cys, Phe, His, Ile, or Trp (C, F, I). Plasma insulin and glucose concentrations from 0 to 2 h after feeding are shown as AUC (a–c and d–f, respectively), and food consumptions for 0–1, 1–2 h after feeding are shown as a cumulative bar chart (g–i). Values are expressed as means ± S.E.M. (n = 5 in each group). Results of statistical analysis are given above the graphs (*P < 0.05; **P < 0.01 vs. 20AA). For the food consumption, cumulative value of 0–2 h after feeding was analyzed statistically