Table 1 Primer sequences used in the quantitative RT-qPCR analysis
From: Postprandial low-grade inflammation does not specifically require TLR4 activation in the rat
Gene | Forward sequence | Reverse sequence |
|---|---|---|
Il-6 | AAGTCGGAGGCTTAATTACATATGTTC | TCATCGCTGTTCATACAATCAGAA |
Il-1β | GCACCTTCTTTTCCTTCATC | GCCGTCTTTCATCACACA |
Tnfα | TGTCTTTGAGATCCATGCCATT | TCGTAGCAAACCACCAAGCA |
Il-10 | CGGGGTGACAATAACTGC | CCTGGGGCATCACTTCTAC |
Pai-1 | GATCTTGACCTTTTGTAGTGCTTGTG | TGGGCATGACTGACATCTTCA |
Mcp-1 | GGCTCAGCCAGATGCAGTTAA | CCAGCCTACTCATTGGGATCA |
Tlr4 | CTGTTGGATGGAAAAGCC | GAGGTCGTTGAGGTTAGAAGC |
Tlr2 | GGGACCTTTGCTATGATGC | CTGTTTTGTGGCTCTTTTCG |
Lpl | GGACTGAGGATGGCAAGCA | GGCAGGGTGAAGGGAATGTT |
Fas | TGC-TCC-CAG-CTG-CAG-GC | GCC-CGG-TAG-CTC-TGG-GTG-TA |
Icam-1 | GAAGACAGCAGACCACTGTGCTT | CTCTGGGAACGAATACACAGTGAT |
Vcam-1 | TGT-GCC-TTG-CGG-ATG-GT | GAA-GTG-TGC-CCG-AAA-TAT-GGA |
Hprt | CTCATGGACTGATTATGGACAGGAC | GCAGGTCAGCAAAGAACTTATAGCC |