Skip to main content

Table 1 Primer sequences used for mRNA quantification by qRT-PCR

From: Maternal high-fat diet impairs glucose metabolism, β-cell function and proliferation in the second generation of offspring rats

Gene name

Primer sequences (5′ to 3′)

Forward

Reverse

Ins1

GTCAAACAGCACCTTTGTGG

CCAGTTGGTAGAGGGAGCAG

Ins2

GTCAAACAGCACCTTTGTGG

TCCAGTTGGTAGAGAGAGCAGA

Pdx1

AAACCGTCGCATGAAGTGGAA

CGAGGTTACGGCACAATCCTG

NeuroD1

GCAAAGGTTTGTCCCAGC

ACGTGGAAGACGTGGGAG

18 s

AAGTTTCAGCACATCCTGCGAGTA

TTGGTGAGGTCAATGTCTGCTTTC

  1. Ins1 Insulin 1, Ins2 Insulin 2, Pdx1 pancreatic and duodenal homeobox factor-1, NeuroD1 Neurogenic differentiation 1
Back to article page

Nutrition & Metabolism

ISSN: 1743-7075

Contact us