An excess dietary vitamin E concentration does not influence Nrf2 signaling in the liver of rats fed either soybean oil or salmon oil

Table 2 Sequences of specific primers used for qPCR

Gene^a	Primer Sequence (5` to 3`)^b		NCBI Accession number
Reference genes
ATP5B	F: GCACCGTCAGAACTATTGCT	R: GAATTCAGGAGCCTCAGCAT	NM_134364.1
CANX	F: CCAGATGCAGATCTGAAGAC	R: CTGGGTCCTCAATTTCACGT	NM_172008.2
MDH1	F: CAGACAAAGAAGAGGTTGCC	R: CGTCAGGCAGTTTGTATTGG	NM_033235.2
Target genes
ATF4	F: AACACAGCCCTTCCACCTCC	R: TGCTCAGCCCTCTTCTTCTGG	NM_024403.2
CAT	F: GTTCAGCGACCGAGGGATTCC	R: TTCCTGTGCAAGTCTTCCTGCC	NM_012520.2
DDITS	F: ACAAGCACCTCCCAAAGCCC	R: TGCTCCTTCTCCTTCATGCGC	NM_001109986.1
DNAJC3	F: AAGGGTCTGTCACTGCTTCTCC	R: TCTCTAAGCCTTCCCGAATCTGC	NM_022232.1
EDEM1	F: ATCCTCGGGTGAATCTGAAGACG	R: TCATAGAAGGAATCCAGCCCAGC	NM_001305279.1
GCLM	F: AGCATCCCTGACATTGAAGCCC	R: GCCAAACCACCACATTCACGC	NM_017305.2
GPX1	F: TCAGTTCGGACATCAGGAGAATGG	R: GGATCGTCACTGGGTGCTGG	NM_030826.4
GSTA1	F: TGCAGCTGGAGTAGAGTTTG	R: ATGGGCACTTGCTGGAACAT	NM_031509.2
HERPUD1	F: ACCGTAGTCATGTACCTGCACC	R: TCAGGGTCCAGCACTTCACG	NM_053523.1
HMOX1	F: AGCATGTCCCAGGATTTGTC	R: TCACCAGCTTAAAGCCTTCC	NM_012580.2
HSPA5	F: TCAGCCCACCGTAACAATCAAGG	R: TCCTCAGCAAACTTCTCGGCG	NM_013083.2
KEAP1	F: CCCTGTGCCTCTATGAGCGT	R: TGCCACTCGTCTCGATCTGG	NM_057152.2
NFE2L2	F: CCCAGCACATCCAGACAGACA	R: GGCTGGGAATATCCAGGGCAA	NM_031789.2
NQO1	F: CCTGGAAGGGTGGAAGAAGCG	R: ATCTGGTTGTCGGCTGGAATGG	NM_017000.3
PDIA4	F: GCAAAGATTGACGCGACCTC	R: GGTGGAGGTGTCCAATCAGG	NM_053849.1
SOD1	F: TATGGTGGTCCACGAGAAAC	R: AATCACACCACAAGCCAAGC	NM_017050.1
SULT1B1	F: TCGCTGGAAATGTGGCCTATGG	R: TGGGCAGATGGGTGTAATTGACC	NM_022513.2
UGT1A6	F: CCCGCTATCGCTCCTTTGGG	R: CAGCCAGGATCACACCACAGG	NM_001039691.2

^a ATP5B ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, ATF4 activating transcription factor 4, CANX calnexin, CAT catalase, DDIT3 DNA damage inducible transcript 3, DNAJC3 DnaJ heat shock protein family (Hsp40) member C3, EDEM1 ER degradation enhancing alpha-mannosidase like protein 1, GCLM glutamate cysteine ligase, modifier subunit, GPX1 glutathione peroxidase 1, GSTA1 glutathione S-transferase alpha 1, HERPUD1 homocysteine inducible ER protein with ubiquitin like domain 1, HMOX1 heme oxygenase 1, HSPA5 heat shock protein family A member 5, KEAP1 kelch like ECH associated protein 1, MDH1 malate dehydrogenase 1, NFE2L2 nuclear factor, erythroid 2 like 2, NQO1 NAD(P)H quinone dehydrogenase 1, PDIA4 protein disulfide isomerase family A, member 4, SOD1 superoxide dismutase 1, SULT1B1 sulfotransferase family 1B member 1, UGT1A6 UDP glucuronosyltransferase family 1 member A6
^b F forward, R reverse

ISSN: 1743-7075