Fig. 4

Knocking down collagen VI inhibits the adipogenesis of 3T3-L1 cells. The fluorescence abundance reached a peak after 72 h of transfection, and the adipogenic inducers were added. Oil red O staining, RNA and protein extractions were performed at D0, D3 and D7 after induction. a The transfection results of the 3T3-L1 with Lenti-GFP and Lenti-ColVI-GFP (MOI = 100). The blue arrows point to the cells with lipid droplets, while the red arrows point to the un-adipogenic cells by Lenti-ColVI-GFP; b Oil red O staining; c mRNA levels of collagen VI and adipogenic-specific genes in the 3T3-L1 cells detected by qPCR. mRNA levels of the target gene are normalized to β-actin. Values are expressed as the mean ± SEM. *P < 0.05, **P < 0.01 versus the control group (n = 3); d Western blotting results at the same stage with mRNA levels. e Relative expression of the target protein