Skip to main content

Advertisement

Table 2 Inflammatory proteins secreted by RAW264.7 cells

From: Z-ligustilide and anti-inflammatory prostaglandins have common biological properties in macrophages and leukocytes

Protein Secretion by unstimulated cells [pg/mL] LPS-induced protein secretion (ratio LPS-stim./unstim.) % inhibition by LIG (25 μM) % inhibition by 15-dPGJ 2 (1.25 μM)
CCL2/MCP-1 4005 65 86 ± 5 ***) 58 ± 39 **)
CCL4/MIP-1β 39,250 7.0 40 ± 13 *) -9 ± 22
CCL5/RANTES 268 219 61 ± 4 **) 2 ± 27
IFN-γ 22 4.2 32 ± 3 **) 24 ± 4 *)
GM-CSF 134 16 94 ± 5 **) 91 ± 2 ***)
IL-1α 9 233 95 ± 4 ***) 50 ± 1 **)
IL-6 28 1442 90 ± 0 ***) 76 ± 15 *)
IL-12p70 84 4.6 38 ± 2 **) 27 ± 7 *)
TNF-α 40 3820 68 ± 5 **) 67 ± 2 **)
  1. RAW264.7 cells were stimulated with LPS and cultured for 24 h in the presence of substances. Secreted proteins were measured by multiparametric analysis (Luminex technology). The LPS-induced increase of secreted proteins is given as ratio of values obtained in stimulated versus unstimulated cells. * p < 0.05; ** p < 0.01; *** p < 0.005 (‘LPS + substance’ treated versus ‘LPS-only’ treated cells)