Z-ligustilide and anti-inflammatory prostaglandins have common biological properties in macrophages and leukocytes

Table 2 Inflammatory proteins secreted by RAW264.7 cells

Protein	Secretion by unstimulated cells [pg/mL]	LPS-induced protein secretion (ratio LPS-stim./unstim.)	% inhibition by LIG (25 μM)	% inhibition by 15-dPGJ ₂ (1.25 μM)
CCL2/MCP-1	4005	65	86 ± 5 ^***)	58 ± 39 ^**)
CCL4/MIP-1β	39,250	7.0	40 ± 13 ^*)	-9 ± 22
CCL5/RANTES	268	219	61 ± 4 ^**)	2 ± 27
IFN-γ	22	4.2	32 ± 3 ^**)	24 ± 4 ^*)
GM-CSF	134	16	94 ± 5 ^**)	91 ± 2 ^***)
IL-1α	9	233	95 ± 4 ^***)	50 ± 1 ^**)
IL-6	28	1442	90 ± 0 ^***)	76 ± 15 ^*)
IL-12p70	84	4.6	38 ± 2 ^**)	27 ± 7 ^*)
TNF-α	40	3820	68 ± 5 ^**)	67 ± 2 ^**)

RAW264.7 cells were stimulated with LPS and cultured for 24 h in the presence of substances. Secreted proteins were measured by multiparametric analysis (Luminex technology). The LPS-induced increase of secreted proteins is given as ratio of values obtained in stimulated versus unstimulated cells. * p < 0.05; ** p < 0.01; *** p < 0.005 (‘LPS + substance’ treated versus ‘LPS-only’ treated cells)

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