Fig. 3

The mRNA and protein expression of bile acid absorption transporters in C57BL/6J mice (n = 5). Total RNA and total protein were extracted from small intestine tissues. mRNA transcription levels were measured using real-time PCR analysis, and protein expression levels were measured using Western blot analysis. The housekeeping gene β-actin was used to normalize expression levels. Critical threshold (Ct) values were compared in a, while relative light density values were compared in c. ^aP < 0.05 versus CR-LCT group; ^bP < 0.05 versus CR group; a. critical threshold; b. section of blots; c. grey-scale analysis