Fig. 2

Protein expression of enzymes operating in hepatic de novo lipogenesis. All data are means ± SEM and were collected from mice after 12-week feeding either control (c), HFD (HF), or n-3 long-chain polyunsaturated fatty acid (LCPUFA)-enriched HFD (HF/n-3). a, b Western blot analyses of (a) AMP-activated protein kinase α (AMPKα) and (b) acetyl-CoA carboxylases (ACC1_2) and their representative phosphorylations in liver (n = 5–6). Separate gels were run for quantification of total AMPKα (62 kDa) and p-AMPKα (Thr172; 62 kDa) and ACC1_2 (265 kDa and 280 kDa), p-ACC1 (Ser79; 257 kDa) and p-ACC2 (Ser219/Ser221; 275–280 kDa), respectively. Shown are the protein expression levels relative to controls and the ratios for the phosphorylated forms to total protein expression levels (normalised values were used). β-ACTIN (42 kDa) or HSP90 (90 kDa) was used for protein normalisation. *p < 0.05, **p < 0.01, ***p < 0.001, significant differences compared to control or between groups as indicated