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Table 8 Effect of vitamin D3 and LPS challenge on serum cytokines of laying hens infected with LPS*

From: Dietary vitamin D3 supplementation protects laying hens against lipopolysaccharide-induced immunological stress

Items

Dietary VD3 levels, IU/kg

 

P-values

0

3000

Saline

LPS

Saline

LPS

SEM

VD3

LPS

Interaction

TNF-α (pg/ml)

134.29

183.78

114.62

168.08

7.53

0.114

< 0.001

0.854

IFN-γ (pg/ml)

66.71

77.66

60.85

67.70

3.09

0.211

0.162

0.741

IL-1β (pg/ml)

15.01

18.65

13.15

17.26

0.60

0.085

< 0.001

0.792

IL-2 (pg/ml)

0.90

1.37

1.05

1.62

0.09

0.154

< 0.001

0.726

IL-4 (pg/ml)

25.95

49.70

24.50

48.36

3.39

0.777

< 0.001

0.992

IL-6 (pg/ml)

90.31

145.60

81.79

124.79

6.47

0.031

< 0.001

0.337

IL-8 (pg/ml)

28.49

48.34

25.96

44.06

2.74

0.400

< 0.001

0.828

IL-10 (pg/ml)

53.71

43.01

37.21

35.35

2.16

0.001

0.066

0.186

IFN-γ/IL-4

2.57

1.56

2.48

1.40

0.26

0.001

0.686

0.729

  1. *Data are presented as mean ± SEM (n = 6 hens /group)
  2. a-c Data in the same row with no common superscript differ significantly (P < 0.05)
  3. SEM standard error of the mean; LPS lipopolysaccharide; TNF-α tumor necrosis factor; IL interleukin; IFN interferon
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Nutrition & Metabolism

ISSN: 1743-7075

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