Fig. 6

Fndc5 protein contents were increased in gastrocnemius muscle tissue of exercised and BCAAs supplemented mice. Similar to what observed for the Fndc5 transcript levels, protein contents of FNDC5 was highest in 20BCAA/Ex group at p < 0.05 as well as Ex group. Data are shown as mean ± SEM (Standard Error of Mean) for each group (n = 6, ●). For better comparison, one of the immunoblots is shown above the diagram. + indicates statistically significant difference with Sed group at p < 0.05, * indicates statistically significant difference with 20BCAA/Ex group at p < 0.05, # indicates statistically significant difference with 60BCAA/Ex group at p < 0.05, & indicates statistically significant difference with Ex group at p < 0.05, $ indicates statistically significant difference with 20BCAA group at p < 0.05, ^ indicates statistically significant difference with 60BCAA group at p < 0.05. Data of western blotting were obtained on triplicate data sets for each sample and the intensity of each bond was quantified by image J. Two-way analysis of variance (ANOVA) and tukey’s post hoc test were used for statistical analysis