Table 1 Primer list
Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) | Annealing temperature (°C) | Accession no. |
|---|---|---|---|---|
Gapdh | TGCCGCCTGGAGAAACC | TGAAGTCGCAGGAGACAACC | 58.6 | NM_008084.2 |
Pgc-1α | CCCTGCCATTGTTAAGACC | TGCTGCTGTTCCTGTTTTC | 52 | NM_008904.2 |
Fndc5 | TCATTGTTGTGGTCCTCTTC | GCTCGTTGTCCTTGATGATA | 60 | NM_027402.3 |
PPARγ | TGAGACCAACAGCCTGAC | GTTCACCGCTTCTTTCAAATC | 58 | NM_001127330.1 |
ATP5a1 | TCCGCTTACATTCCAACA | ACACAGACAAACCCACAT | 54 | NM_007505.2 |
ATP5b | GGTAGCGTTGGTATATGG | CTCCTGGTCTCTGAAGTA | 54 | NM_016774.3 |
Cox4i1 | ATGGGAGTGTTGTGAAGAGT | CATCAGGCAAGGGGTAGT | 54 | NM_009941.3 |
Tfam | CTTCAACCACCACACCACT | AATCTCTAAGCCTCCTCAATACAA | 56 | NM_009360.4 |
Sirt1 | GGCAGTAACAGTGACAGT | CTCTCCGTATCATCTTCCAA | 61 | NM_001159589.2 |