Fig. 1

Characterization of Tnnt3 mRNA, protein, and splice form expression in differentiating L6 myotubes. L6 myotubes were collected at the onset of differentiation (D0) or eight days post differentiation (D8) as described in Methods. (a) Micrograph of D8 L6 myotubes. (b) Tnnt3 mRNA abundance was assessed by qRT-PCR and normalized to Gapdh. (c) TNNT3 protein expression was assessed by Western blot analysis. Protein staining of the Western blot membrane is shown to demonstrate equal protein loading. Capillary electrophoresis tracings of Tnnt3 splice forms from (d) D0 or (e) D8 of differentiation as assessed by fragment analysis of Tnnt3 PCR amplicons. Black and gray tracings represent Tnnt3 splice forms and internal size standards, respectively. Peak height is proportional to the splice form relative abundance. Data in (b) are presented as means ± SEM from three independent experiments using three replicates per time point. Statistically different means as assessed by Student’s t-test are denoted with an asterisk (*) (p ≤ 0.05)